Q: The cells
on this IFA slide all appear positive. Is this possible?
A: If all the
cells are positive this is likely to be some type of an
anti-cellular reaction. The percent positive should be approximately
the same as seen with the positive control.
Q: When only
two or three cells are seen fluorescing is this a positive?
A: No, the
percent of positive cells seen should be at least as high as the
number of positive cells seen with the positive control at its
endpoint.
Q: Why is
the positive control for this IFA kit barely positive at the
screening dilution?
A:
The positive controls are bottled at the screening
dilution and can be titered for an endpoint from this dilution. The
control should be used in the first well without further
dilution.
Q: Can the
VCA IgG slides be used for IgM testing?
A: We do not recommend this
these slides are not optimized for IgM testing. the VCA IgM RIFA kit
RIF0600M was specifically designed for VCA IgM testing. |
